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goat anti human cd55 antibody  (R&D Systems)


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    Structured Review

    R&D Systems goat anti human cd55 antibody
    Goat Anti Human Cd55 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+human+cd55/Human+CD55%2FDAF+Antibody/us10813977-495-38-43
    Average 94 stars, based on 13 article reviews
    goat anti human cd55 antibody - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Macroscopical, Histological, and In Vitro Characterization of Nonosteoarthritic Versus Osteoarthritic Hip Joint Cartilage
    Article Snippet: .. The primary antibodies (rabbit anti-human C3aR [1:30, Assay Biotechnology], mouse anti-human C5aR [1:100, GeneTex, Biozol], mouse anti-human CD46, CD59 [1:20 and 1:40, both AbD Serotec], and goat anti-human CD55 [1:40, R&D Systems]) were diluted in blocking buffer containing 0.1% Triton X-100 (Sigma-Aldrich) and incubated overnight at 4 °C in a humidified chamber. .. After rinsing three times with TBS, the labeled secondary antibody (donkey anti-rabbit or donkey anti-mouse-Alexa488 or -Alexa555, 10 mg/mL; Life Technologies Corporation) was diluted 1:200 in blocking buffer containing 0.1% Triton X-100 and 0.1 μg/μL DAPI and incubated for one hour at RT in a dark room.

    Article Title: Macroscopical, Histological, and In Vitro Characterization of Nonosteoarthritic Versus Osteoarthritic Hip Joint Cartilage
    Article Snippet: .. The primary antibodies (rabbit anti-human C3aR [1:30, Assay Biotechnology], mouse anti-human C5aR [1:100, GeneTex, Biozol], mouse anti-human CD46, CD59 [1:20 and 1:40, both AbD Serotec], and goat anti-human CD55 [1:40, R&D Systems]) were diluted in blocking buffer containing 0.1% Triton X-100 (Sigma-Aldrich) and incubated overnight at 4 °C in a humidified chamber. .. After rinsing three times with TBS, the labeled secondary antibody (donkey anti-rabbit or donkey anti-mouse-Alexa488 or -Alexa555, 10 mg/mL; Life Technologies Corporation) was diluted 1:200 in blocking buffer containing 0.1% Triton X-100 and 0.1 μg/μL DAPI and incubated for one hour at RT in a dark room.

    Incubation:

    Article Title: Macroscopical, Histological, and In Vitro Characterization of Nonosteoarthritic Versus Osteoarthritic Hip Joint Cartilage
    Article Snippet: .. The primary antibodies (rabbit anti-human C3aR [1:30, Assay Biotechnology], mouse anti-human C5aR [1:100, GeneTex, Biozol], mouse anti-human CD46, CD59 [1:20 and 1:40, both AbD Serotec], and goat anti-human CD55 [1:40, R&D Systems]) were diluted in blocking buffer containing 0.1% Triton X-100 (Sigma-Aldrich) and incubated overnight at 4 °C in a humidified chamber. .. After rinsing three times with TBS, the labeled secondary antibody (donkey anti-rabbit or donkey anti-mouse-Alexa488 or -Alexa555, 10 mg/mL; Life Technologies Corporation) was diluted 1:200 in blocking buffer containing 0.1% Triton X-100 and 0.1 μg/μL DAPI and incubated for one hour at RT in a dark room.

    Article Title: Complement gene expression is regulated by pro-inflammatory cytokines and the anaphylatoxin C3a in human tenocytes.
    Article Snippet: Interplay between complement factors, regulatory proteins, anaphylatoxins and cytokines could be involved in tendon healing and scar formation.. The expression and regulation of complement factors by cytokines or anaphylatoxins are completely unclear in tendon.. Hence, the gene expression of the anaphylatoxin receptors C3aR, C5aR and cytoprotective complement regulatory proteins (CRPs) was analysed in human tendon, cultured primary tenocytes and to directly compare the general expression level, additionally in human leukocytes.

    Article Title: Macroscopical, Histological, and In Vitro Characterization of Nonosteoarthritic Versus Osteoarthritic Hip Joint Cartilage
    Article Snippet: .. The primary antibodies (rabbit anti-human C3aR [1:30, Assay Biotechnology], mouse anti-human C5aR [1:100, GeneTex, Biozol], mouse anti-human CD46, CD59 [1:20 and 1:40, both AbD Serotec], and goat anti-human CD55 [1:40, R&D Systems]) were diluted in blocking buffer containing 0.1% Triton X-100 (Sigma-Aldrich) and incubated overnight at 4 °C in a humidified chamber. .. After rinsing three times with TBS, the labeled secondary antibody (donkey anti-rabbit or donkey anti-mouse-Alexa488 or -Alexa555, 10 mg/mL; Life Technologies Corporation) was diluted 1:200 in blocking buffer containing 0.1% Triton X-100 and 0.1 μg/μL DAPI and incubated for one hour at RT in a dark room.

    Staining:

    Article Title: Complement gene expression is regulated by pro-inflammatory cytokines and the anaphylatoxin C3a in human tenocytes.
    Article Snippet: Interplay between complement factors, regulatory proteins, anaphylatoxins and cytokines could be involved in tendon healing and scar formation.. The expression and regulation of complement factors by cytokines or anaphylatoxins are completely unclear in tendon.. Hence, the gene expression of the anaphylatoxin receptors C3aR, C5aR and cytoprotective complement regulatory proteins (CRPs) was analysed in human tendon, cultured primary tenocytes and to directly compare the general expression level, additionally in human leukocytes.



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    R&D Systems goat anti human cd55
    Expression of complement receptors (C5aR and C3aR) and CRPs (CD46, <t>CD55,</t> and CD59) in cartilage in situ. FNF and OA cartilage sections immunolabeled for ( A 1–2 ) C5aR, ( B 1–2 ) C3aR, ( C 1–2 ) CD46, ( D 1–2 ) CD55, and ( E 1–2 ) CD59. Notes: Cell nuclei were counterstained using DAPI (blue). Scale bar: 200 µm. Negative controls (B 3 ) performed by using only the secondary antibody revealed no unspecific staining.
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    Expression of complement receptors (C5aR and C3aR) and CRPs (CD46, <t>CD55,</t> and CD59) in cartilage in situ. FNF and OA cartilage sections immunolabeled for ( A 1–2 ) C5aR, ( B 1–2 ) C3aR, ( C 1–2 ) CD46, ( D 1–2 ) CD55, and ( E 1–2 ) CD59. Notes: Cell nuclei were counterstained using DAPI (blue). Scale bar: 200 µm. Negative controls (B 3 ) performed by using only the secondary antibody revealed no unspecific staining.
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    Image Search Results


    Expression of complement receptors (C5aR and C3aR) and CRPs (CD46, CD55, and CD59) in cartilage in situ. FNF and OA cartilage sections immunolabeled for ( A 1–2 ) C5aR, ( B 1–2 ) C3aR, ( C 1–2 ) CD46, ( D 1–2 ) CD55, and ( E 1–2 ) CD59. Notes: Cell nuclei were counterstained using DAPI (blue). Scale bar: 200 µm. Negative controls (B 3 ) performed by using only the secondary antibody revealed no unspecific staining.

    Journal: Clinical Medicine Insights. Arthritis and Musculoskeletal Disorders

    Article Title: Macroscopical, Histological, and In Vitro Characterization of Nonosteoarthritic Versus Osteoarthritic Hip Joint Cartilage

    doi: 10.4137/CMAMD.S29844

    Figure Lengend Snippet: Expression of complement receptors (C5aR and C3aR) and CRPs (CD46, CD55, and CD59) in cartilage in situ. FNF and OA cartilage sections immunolabeled for ( A 1–2 ) C5aR, ( B 1–2 ) C3aR, ( C 1–2 ) CD46, ( D 1–2 ) CD55, and ( E 1–2 ) CD59. Notes: Cell nuclei were counterstained using DAPI (blue). Scale bar: 200 µm. Negative controls (B 3 ) performed by using only the secondary antibody revealed no unspecific staining.

    Article Snippet: The primary antibodies (rabbit anti-human C3aR [1:30, Assay Biotechnology], mouse anti-human C5aR [1:100, GeneTex, Biozol], mouse anti-human CD46, CD59 [1:20 and 1:40, both AbD Serotec], and goat anti-human CD55 [1:40, R&D Systems]) were diluted in blocking buffer containing 0.1% Triton X-100 (Sigma-Aldrich) and incubated overnight at 4 °C in a humidified chamber.

    Techniques: Expressing, In Situ, Immunolabeling, Staining